FIGURE 5.

Effect of hydrogen peroxide on Anabaena and Synechocystis 2-Cys Prx overoxidation in vivo. Cultures grown at 50 μE m⁻² s⁻¹ to a concentration of 5 μg of chlorophyll ml⁻¹ were incubated for 15 min with hydrogen peroxide at the indicated concentrations. Cultures treated with 0.5 mm hydrogen peroxide were also harvested 2 and 4 h after the addition of hydrogen peroxide. Western blot analysis was performed using antibodies raised against rice and Synechocystis 2-Cys Prx for Anabaena and Synechocystis samples, respectively. Molecular mass markers, in kDa, are indicated on the left. mon, monomer; dim, dimer.