FIGURE 10.

Assessment of the contribution of endoplasmic reticulum to the L. donovani-containing phagosome. Cryosections of human polymorphonuclear neutrophils (PMN) incubated with L. donovani parasites (LD) for 3 h were immunogold labeled with the endoplasmic reticulum-resident PDI, using specific antibodies. The upper panel shows a just internalized L. donovani parasite (LD), where the condensation of actin (electron-dense) below the phagosome membrane is visible. After labeling with anti-PDI antibodies, the endoplasmic reticulum cisternae (er) from the PMN were extensively labeled, whereas the label associated with phagosomes was negligible. The nucleus of the neutrophil is shown, n. Bar, 200 nm.