FIGURE 5.

Subcellular localization of specific granules after L. major internalization in human neutrophils. Cryosections of human polymorphonuclear neutrophils (PMN) incubated with L. major LV39 parasites for 3 h were immunogold labeled with lactoferrin (Lf), using specific antibodies. L. major LV39 parasite was shown in the phagosome. After labeling with anti-lactoferrin antibody, almost no label was detected in the phagosome, whereas abundant labeled lactoferrin-positive granules were observed in the cytoplasm (arrows pointed to clusters of positive granules). The nucleus of the neutrophil is indicated, n. Bar, 500 nm.