FIGURE 8.

Localization of azurophilic granule membrane marker CD63 in L. donovani-containing phagosome. Cryosections of human neutrophils, incubated with L. donovani parasites (LD) for 3 h, were immunogold labeled with anti-CD63 antibody as a membrane marker for azurophilic granules. Lower panel shows fusion of CD63-positive azurophilic granules (g, arrows) with the parasite-containing phagosome. In another area, the CD63-positive granular membrane (arrow) is inserted in the phagosome membrane. The upper panel shows the CD63 label on the phagosome membrane (arrows) containing a L. donovani parasite (LD) with a flagellar pocket (fp). Bar, 200 nm.