FIGURE 5.

Recruitment of arrestin2 to CCR5 is facilitated by NHERF1. a, HEK293 cells were co-transfected with cDNAs encoding NHERF1 WT or PDZ1-PDZ2, along with CCR5-Venus1 and CCR5-Venus2. Cells were then lysed, and immunoprecipitations using an antibody directed against GFP were performed. An immunoblot was then performed using an antibody directed against arrestin2. b, histogram representation of the results obtained by immunoblotting. *, p < 0.05; **, p < 0.01 compared with negative controls using two-tailed paired Student's t test. Results are representative of three independent experiments.