FIGURE 2.
Effects of blockage of Kv7 currents on the resting membrane potential (Vrest). A, shown is characteristic Vrest of a 0.5-month-old apical SGN under control conditions. B, application of linopirdine (lino, 10 μm)-induced membrane depolarization in basal 0.5-month-old basal SGN (upper panel). The effect of the drug was reversible after washout. C depicts the effect of linopirdine on 0.5-month-old (mo) (2 week (wk)) apical (left panel) and basal (right panel). D, summary data of changes in Vrest in apical and basal SGNs of 0.5-month-old mice (n = 11; *, p < 0.05; **, p < 0.01). con, control. E, examples of raw traces showing changes in Vrest after application of linopirdine in apical (left panel) and basal SGNs isolated from 3- to 4-month-old mice. F, corresponding summary data obtained from 3- to 4-month-old SGNs (n = 7). G, linopirdine had very little effect on apical neurons isolated from 17-month-old mice compared with age-matched basal SGNs. H, summary data of the effects linopirdine on Vrest of apical and basal 17-month-old neurons. I and J show changes in Vrest in different aged group of neurons at apical (I) and basal (J) aspects of the cochlea.