FIGURE 6.
Changes in Ca2+i after blockade and enhancement of Kv7-mediated current by linopirdine and retigabine, respectively. SGNs were loaded with Fluo 4/AM, and the changes in [Ca2+]i were monitored by measuring the fluorescence intensity. A, effects of 10 μm linopirdine showing a steady and sustained rise in [Ca2+]i. Shown is the effect of linopirdine and partial recovery after washout of the drug. B, to determine the effects of retigabine, we depolarized Fluo 4-loaded SGNs with 10 mm KCl before application of 10 μm retigabine in 10 mm KCl solution. Retigabine reversed the effects of high external K+ by reducing [Ca2+]i. Summary data were obtained from n = 5 (*, p < 0.5; **, p < 0.01).