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. 2010 Aug 31;285(45):34718–34728. doi: 10.1074/jbc.M110.126441

FIGURE 4.

FIGURE 4.

Pituitary dysmorphology, hypoplasia, and hormone deficiencies in the Pitx2-Cre/Dicer1 conditional knock-out (cKO) mouse. A, hematoxylin and eosin-stained pituitaries reveal an abnormal ventral bulge in the anterior lobe and hypoplasia in Pitx2-Cre/Dicer1 mutant pituitaries compared with wild type mice from E14.5 to P0. B, hormone deficiency in P0 Pitx2-Cre/Dicer1 mutant mice. Immunohistochemistry in P0 pituitaries demonstrated decreased expression of GH, PRL, TSHβ, and LHβ in the mutant pituitary, whereas ACTH/POMC expression is similar to wild type. C, immunohistochemistry using a Pit-1 antibody reveals decreased Pit-1 expression at E15.5 and P0 in mutant pituitaries compared with wild type pituitaries. D, immunohistochemistry using a Lef-1 antibody demonstrates increased Lef-1 expression in E15.5 and P0 mutant pituitaries compared with wild type pituitaries. WT, wild type mice; scale bars, 100 μm. All histology panels are sagittal sections of embryonic or P0 pituitary.