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. 2010 Sep 7;285(45):34793–34802. doi: 10.1074/jbc.M110.150227

TABLE 2.

Substrate specificity

The reaction was carried out as described under “Experimental Procedures.”

Substrate Specific activity
units/mg
Cyclohexyl isocyanide 0.82 ± 0.01
Benzyl isocyanide 5.65 ± 0.06
1-Pentyl isocyanide 2.97 ± 0.09
tert-Butyl isocyanide 0.60 ± 0.01
Methyl isocyanoacetate 3.50 ± 0.02
Ethyl isocyanoacetate 5.90 ± 0.10
Isocyanomethyl phosphonic acid diethyl ether 0.00004 ± 0.00003