TABLE 3.
Effects of various compounds on the activity of isonitrile hydratase
Each compound was added to the standard reaction mixture without the substrate, and then assaying of the enzyme was performed after adding the substrate. The final concentration of the compounds was 1 mm, unless otherwise stated.
| Inhibitor | Relative activity |
|---|---|
| % | |
| None | 100 |
| LiCl, NaCl, MgCl, CaCl2, MnCl2, RbCl, CsCl, SrCl2 | 95–103 |
| Na2MoO4 | 94.0 |
| FeSO4 (0.25 mm) | 92.4 |
| Pb(NO3)2 | 88.1 |
| FeCl3 | 80.3 |
| AlCl3 | 78.6 |
| SnCl2 | 58.3 |
| ZnCl2 | 48.1 |
| CdCl2 | 28.4 |
| CoCl2 | 10.5 |
| NiCl2 | 5.0 |
| HgCl2 | 0 |
| CuCl2 | 0 |
| AgNO3 | 0 |
| Iodoacetate | 96.4 |
| 5,5′-Dithiobis-2-nitrobenzoate | 65.1 |
| N-Ethylmaleimide | 51.9 |
| p-Chloromercuribenzoate | 0.2 |
| Hydroxylamine | 108 |
| Phenylhydrazine | 98.2 |
| Semicarbazide | 100 |
| Aminoguanidine | 97.9 |
| α,α-Dipyridyl | 105 |
| o-Phenanthroline | 108 |
| 8-Hydroxyquinoline | 73.9 |
| EDTA | 108 |
| Diethyldithiocarbamate | 103 |
| NaN3 | 95.8 |
| KCN | 100 |
| Dithiothreitol | 110 |
| 2-Mercaptoethanol | 98.8 |
| Na2S2O4 | 105 |
| H2O2 | 55.0 |
| Ammonium persulfate | 94.8 |
| Phenylmethanesulfonyl fluoride | 50.6 |
| Diisopropyl fluorophosphate | 98.5 |