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Immunoprecipitation and Western blotting of total cellular proteins. Total cellular proteins from non-treated and FX-S195A (175 nm, 30 min)-treated EA.hy926 cells were immunoprecipitated with anti-PAR-1 (A), anti-caveolin-1 (B), anti-EPCR (C), and anti-PAR-2 (D) antibodies, separated on SDS-PAGE and immunoblotted (IB) using the same four antibodies as described under “Experimental Procedures.”
