FIGURE 2.

Dose-response signaling curves of the three GpHRs, WT, and mutant forms in β-strand 4. Representative cAMP responses are shown for the WT receptors and Ala replacements at Asn¹²⁹ (FSHR), Asn¹³² (LHR), and Asn¹³⁵ (TSHR) upon receptor activation with the cognate hormones (hCG being used to activate LHR; h is human). All assays of intracellular cAMP were done by RIA with HEK 293 cells.