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Saturation binding curves of WT and mutant forms of FSHR and LHR with Ala replacements at two positions in β-strand 5. Representative binding curves are shown for the WT receptors and mutant FSHR (D150A and D153A) and LHR (E154A and D157A) forms. All binding studies were done using 125I-labeled FSH and 125I-labeled CG with HEK 293 cells.
