TABLE 1.
Summary of expression, binding, and signaling results for β-strand 2 mutants in HEK 293 cells
| Mutant | Bmax | Kd (mutant/WT) | EC50 (mutant/WT) | Rmax | Q (mutant/WT) |
|---|---|---|---|---|---|
| % WT | % WT | ||||
| FSHR | |||||
| K74A (n = 5) | 87 ± 23 | 0.8 ± 0.2 | 1.0 ± 0.2 | 84 ± 11 | 0.9 ± 0.2 |
| K74E (n = 2) | 102 ± 24 | 0.8 ± 0.3 | 0.7 ± 0.2 | 210 ± 60 | 2.4 ± 0.1 |
| S78A (n = 5) | 66 ± 26 | 0.9 ± 0.3 | 1.4 ± 0.3 | 89 ± 25 | 0.9 ± 0.1 |
| LHR | |||||
| K77A (n = 4) | 143 ± 56 | 1.1 ± 0.2 | 1.0 ± 0.1 | 143 ± 51 | 1.0 ± 0.04 |
| K77E (n = 4) | 159 ± 52 | 1.3 ± 0.5 | 1.7 ± 0.3 | 228 ± 42 | 0.9 ± 0.1 |
| S81A (n = 4) | 217 ± 23 | 1.4 ± 0.3 | 1.3 ± 0.3 | 165 ± 42 | 0.9 ± 0.03 |
| TSHR | |||||
| R80A (n = 3) | a | b | 0.8 ± 0.1 | 70 ± 18 | b |
| R80E (n = 3) | a | b | 0.9 ± 0.3 | 104 ± 23 | b |
| S84A (n = 3) | a | b | 0.5 ± 0.1 | 145 ± 18 | b |
a Data were not determined by binding (see Table 6).
b Data were not determined.