TABLE 5.
Summary of expression, binding, and signaling results for β-strand 8 mutants in HEK 293 cells
| Mutant | Bmax | Kd (mutant/WT) | EC50 (mutant/WT) | Rmax | Q (mutant/WT) |
|---|---|---|---|---|---|
| % WT | % WT | ||||
| FSHR | |||||
| D224A (n = 4) | 39 ± 22 | 1.8 ± 0.8 | 3.1 ± 0.4 | 41 ± 30 | 0.7 ± 0.7 |
| D224K (n = 2) | 84 ± 8 | 1.3 ± 0.1 | a | a | a |
| S226A (n = 3) | 5 ± 1 | b | b | b | b |
| LHR | |||||
| D228A (n = 3) | 37 ± 8 | 4.0 ± 1.5 | 3.9 ± 1.3 | 91 ± 13 | 2.6 ± 0.3 |
| D228K (n = 3) | 80 ± 24 | 5.3 ± 1.8 | >20 | a | a |
| S230A (n = 4) | 95 ± 19 | 0.7 ± 0.1 | 1.5 ± 0.3 | 244 ± 49 | 2.1 ± 0.1 |
| TSHR | |||||
| D232A (n = 3) | c | d | 4.0 ± 1.0 | 52 ± 20 | d |
| D232K (n = 2) | c | d | a | a | d |
| S234A (n = 3) | c | d | 1.3 ± 0.1 | 90 ± 15 | d |
a Signaling was too low to permit measurement of EC50 and Q.
b Expression was too low to permit accurate measurements.
c Data were not determined (see Table 6).
d Data were not determined.