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. 2010 Sep 1;285(45):34960–34971. doi: 10.1074/jbc.M110.150839

FIGURE 4.

FIGURE 4.

Mutational analysis of Stbd1 expressed in COS M9 cells. Mutated hStbd1 with a C-terminal HA tag was expressed in COS M9 cells and immunostained with anti-HA antibodies (red). A, full-length human Stbd1. B, deletion of the N-terminal hydrophobic segment (ΔN24-HA). C, deletion of the N-terminal hydrophobic segment and the putative leucine zipper (ΔN90-HA). D, deletion of the C-terminal carbohydrate binding domain (ΔC96-HA). E, mutation of conserved Trp of CBM20 domain to Gly (W293G). F, mutation of conserved Trp of CBM20 domain to Leu (W293L). Nuclei were stained with Hoechst (blue). Scale bar, 20 μm.