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. 2010 Sep 1;285(45):35029–35038. doi: 10.1074/jbc.M110.165506

FIGURE 2.

FIGURE 2.

RASSF5 interacts with MST1 and other components of Hippo signaling pathway. A, U2OS cells cotransfected with FLAG-RASSF5 and either MST1, WW45, LATS1, or YAP1 were immunoprecipitated (IP) with either FLAG, MST1, WW45, LATS1, or YAP1 antibodies and analyzed by Western blotting (WB). B, 4-week-old mouse brain lysates were immunoprecipitated with anti-RASSF5, anti-MST1, or anti-YAP1 antibody and analyzed by immunoblotting. C, U2OS cells were transfected with control or MST1, WW45, LATS1, or YAP1 siRNA and treated with varying concentrations of TNF-α along with cycloheximide. Cell survival was measured as described.