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. 2010 Sep 1;285(45):35029–35038. doi: 10.1074/jbc.M110.165506

FIGURE 4.

FIGURE 4.

Inactivation of Rassf5 by gene targeting. A, a scheme of Rassf5 targeting strategy. Homologous recombination will delete exons 3–5 and insert the neomycin gene. The triangles indicate LoxP sequences. B, Southern blot analysis of HindIII-digested mouse ES genomic DNA. Wild type (5.6-kb) and targeted (13.8-kb) alleles are indicated. C, PCR analysis of tail DNA. Wild type (177 bp) and targeted (228 bp) bands are indicated. D, mouse brain lysates from 4-week-old Rassf5 wild type and mutant animals or Rassf5+/+ and Rassf5−/− MEFs were analyzed by immunoblotting with anti-RASSF5 antibody. Anti-β-actin was used for loading control. KO, knock-out.