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. 2010 Sep 1;285(45):35029–35038. doi: 10.1074/jbc.M110.165506

FIGURE 6.

FIGURE 6.

Rassf5-deficient mice are resistant to TNF-α-induced apoptosis. A, Rassf5 wild type (n = 7) and mutant mice (n = 4) were injected via tail vein with d-galactosamine and TNF-α. The time of death was recorded for each mouse. B, Rassf5 wild type and mutant mice were injected with TNF-α as in A, and all of the animals were sacrificed at 6 h before death (n = 3 for each genotype). Paraffin-embedded sections of liver were analyzed by H & E and TUNEL staining. Control liver (Rassf5+/−) from an uninjected animal is shown. C, positive TUNEL-stained cells (from B) were counted from at least seven randomly chosen fields, and the average number of TUNEL-positive cells/field is shown (n = 3 for each genotype). D, liver extracts from Rassf5 wild type and mutant TNF-α-treated animals (n = 3 for each genotype) were analyzed by Western blotting with Pro-CASP3, MST1, phospho-H2B, H2B, and α-tubulin antibodies. H&E, hematoxylin and eosin.