FIGURE 7.

Rassf5-deficient MEFs are predisposed to K-RasG12V-induced transformation. A, soft agar assay. Immortalized Rassf5^+/+ and Rassf5^−/− MEFs stably expressing empty vector or K-RasG12V were grown in soft agar for 21 days, and the colonies were counted. The experiment was performed in triplicate and repeated three times using two independently derived MEFs. B, representative images taken under phase-contrast microscope of immortalized Rassf5^+/+ and Rassf5^−/− MEFs stably expressing K-RasG12V are shown. C, immortalized Rassf5^+/+ and Rassf5^−/− MEFs stably expressing empty vector or K-RasG12V were injected subcutaneously into nude mice, and the animals were sacrificed at 3–5 weeks after injection. The tumors were dissected and weighed.