FIGURE 6.
Effects of Ned-19 on TPC2. a, a typical experiment illustrating TPC2 channels gating in the bilayer in the presence of 200 μm luminal Ca2+ (K+ is the permeant ion), pH 7.2. Cytosolic NAADP (10 nm) followed by sequential increments in cytosolic Ned-19 levels was applied as indicated. Po values are shown above the relevant trace. The bottom trace shows the effect of washing out both compounds from the cis chamber; only the effects of Ned-19 are reversible. b, concentration dependence of Ned-19 effects in the presence of NAADP (*, p < 0.05).