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. 2010 Aug 30;285(45):35068–35078. doi: 10.1074/jbc.M110.146126

FIGURE 2.

FIGURE 2.

Expression of recombinant Lm3B11 by HEK-293 cells and its purification. A, conditioned medium of Lm3B11-HEK cells was separated by SDS-PAGE on 3–7.5% gradient gels under nonreducing conditions and then analyzed by immunoblotting for the laminin α3B, β1, and γ1 chains. Arrows indicate the immunoreactive bands corresponding to the Lm3B11 heterotrimer or the β1γ1 heterodimer. B, recombinant Lm3B11 purified from the Lm3B11-HEK conditioned medium was analyzed by SDS-PAGE under nonreducing conditions (left panel, −ME) or reducing conditions (right panel, +ME). Separated proteins were directly stained with Coomassie Brilliant Blue dye (CBB) or identified by immunoblotting for the laminin chains. Arrows indicate identified laminin chains with apparent molecular sizes in kDa. ME, 2-mercaptoethanol.