FIGURE 7.

Binding of wild type galectin-3 and mutants to unprimed and TNFα-primed neutrophils. FITC-labeled galectin was incubated with freshly prepared neutrophils that had been pretreated by incubation for 30 min at 37 °C without (white bars) or with TNFα (gray bars) and at 4 °C without TNFα (black bars) as a control to prevent granule mobilization. The amount of bound galectin was measured by flow cytometry and is given as the mean and S.E. of the geometric mean of fluorescence intensity (y axis) from three experiments on different batches of neutrophils. The p values were calculated from paired one-tailed t tests; paired, to discount the variability between the neutrophils, and one-tailed, to specifically test the hypothesis of an increased binding after incubation with TNFα.