FIGURE 4.

POL30-dependent histone modifications influence silencing. A, map of HMR::ADE2. B and C, overlapping silencing defects in histone H3 K9R and pol30-8 mutants. D, overlapping silencing defects in histone H3 K9R and cac1Δ double mutants. Colony color assays were used to monitor silencing of the ADE2 gene integrated at HMR. Cells were grown logarithmically, plated onto rich medium (YPD), incubated at 30 °C for 2 days, and then stored at 4 °C for 3 days prior to acquiring images (see “Experimental Procedures”).