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Loss of H3-K9 acetylation restores silencing at HMRae**. MATα HMRae** strains expressing the indicated histone H3 mutants were grown on minimal medium with supplements for 24 h at 30 °C and then replicated to rich medium (YPD) or to MATa lawns (JRY2726) on minimal medium and incubated for 2 days at 30 °C. Restoration of silencing prevents expression of a1 from HMRae**, thereby enabling the MATα cells to mate and grow as diploids on minimal medium.
