TABLE 2.
pol30 mutants are defective in establishing silencing in the absence of DNA replication
| Arrest | HMRa | Relative efficiency of a1 mRNA expressionb |
||
|---|---|---|---|---|
| POL30 | pol30-8 | pol30-79 | ||
| G1 | Not replicated | 1 | 1 | 1 |
| G2/M | Not replicated | 0.16 ± 0.092 | 0.32 ± 0.075 | 0.49 ± 0.17 |
| G1 | Replicated | 1 | 1 | 1 |
| G2/M | Replicated | 0.15 ± 0.031 | 0.41 ± 0.20 | 0.36 ± 0.13 |
a HMR was either excised in G1 (Not replicated) or left within the chromosome (Replicated).
b Establishment of silencing was monitored in POL30 and pol30 cells. For each strain, the relative level of a1 mRNA expressed in G1 and in G2/M was normalized to an internal control transcript, SCR1. The data have been normalized to the ratios observed during the G1 arrest, and the levels in G2/M are expressed relative to those in G1 to combine data from independent experiments: mean ± S.D., n = 3 (11).