TABLE 3.

Acetylation of Lys on chromatin-associated histone H3

Strain	Relative levels of H3-K9aca
POL30	100
pol30-8	46 ± 2.8
pol30-6	87 ± 19
pol30-79	96 ± 50
cac1Δ	39 ± 3.3
asf1Δ	17 ± 2.0
cac1Δasf1Δ	14 ± 5.0
cdc44-5	75 ± 18
sas2Δ	100 ± 22

^a The level of chromatin-associated H3-K9ac relative to chromatin-associated H3 in each strain was determined by quantitative Western blot analysis as described under “Experimental Procedures” and was expressed as a percentage of that observed in POL30 cells. Data were calculated as (H3-K9ac/H3)_mutant/(H3-K9ac/H3)_POL30 × 100, where mutant = indicated strain, mean ± S.D., n = 3 to 6 (except n = 2 for sas2Δ).