TABLE 4.

Acetylation of Lys-16 on chromatin-associated histone H4

Strain	Relative levels of H4-K16aca
POL30	100
pol30-8	69 ± 9.5
pol30-6	42 ± 3.4
pol30-79	67 ± 13
cac1Δ	59 ± 12
asf1Δ	70 ± 13
cac1Δasf1Δ	66 ± 16
cdc44-5	65 ± 24
sas2Δ	20 ± 3.1

^a The level of chromatin-associated H4-K16ac relative to chromatin-associated H3 in each strain was determined by quantitative Western blot analysis as described under “Experimental Procedures” and was expressed as a percentage of that observed in POL30 cells. Data were calculated as [(H4-K16ac/H3)_mutant/(H4-K16ac/H3)_POL30] × 100, where mutant = indicated strain, mean ± S.D., n = 3 (except n = 2 for sas2Δ).