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. 2010 Sep 7;285(45):35188–35195. doi: 10.1074/jbc.M110.141200

FIGURE 2.

FIGURE 2.

Effect of indomethacin on nanoclusters in epithelial cell plasma membrane. The lifetime of GFP in BHK cells expressing GFP-tH or GFP-tK alone, or coexpressing GFP-tH and RFP-tH, or GFP-tK and RFP-tK, in the presence or absence of indomethacin, was measured in a wide field FLIM microscope. Data were collected from multiple single cells. GFP lifetime is shown as mean ± S.E. (n = 50–100 cells from three independent experiments). Significant differences are indicated (*, p ≪0.05; p = 8.6 × 10−5 for 100 μm indomethacin, p = 0.00014 for 500 μm indomethacin, p = 0.018 for 750 μm indomethacin and p = 4.2 × 10−11 for 1.5 mm indomethacin). The lower panel contains FLIM images from a representative experiment, showing of BHK cells expressing GFP-tH alone, or co-expressing GFP-tH and RFP-tH and treated with 1.5 mm indomethacin. Scale bar: 20 μm.