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Functional interaction between Ppk11 and SIN. A and B, localization of Ppk11 in the SIN mutant. The indicated SIN mutants having ppk11+:GFP:kanr grown in YES5 medium at 25 °C were shifted to 36 °C, incubated for 4 h, and fixed (A). Cells were fixed and stained with DAPI for visualizing the nucleus. The cdc15 mutant having ppk11+:GFP:kanr and sad1+:dsRED:LEU2 (TG68-1D) grown at 25 °C was shifted to 36 °C and incubated for 4 h (B). Bars indicate 5 μm.
