TABLE 1.

Screening data for mutant cycle analysis. Peptide inhibition of wild-type or α subunit mutant ENaC was determined at the indicated [peptide]. Mutant channel inhibition is expressed relative to wild-type channel inhibition for the same peptide. Values are expressed as mean (n = 2–3).

	Inhibition
	LPHPLQRL (10 μm)	APHPLQRL (30 μm)	LGHGLQRL (50 μm)	LPKPLQRL (50 μm)	LPHPLARL (10 μm)	LPHPLQAL (10 μm)	LPHPLQRA (50 μm)
	%
Wild-type	77.22	52.19	51.22	42.24	51.22	49.64	41.82
Inhmut/Inhwild type
G252W	0.15				0.14	0.23	0.37
Q254W	0.39				0.34	0.30	0.70
L255W	0.46			0.20	0.14	0.21	0.19
C256W	0.49			0.40	0.25	0.17	0.50
T267W	0.11			0.13	−0.02	0.11	0.02
A274W	0.52	0.41	0.56	0.52	0.37	0.36	0.31
V275W	0.43	0.35	0.41	0.31	0.21	0.23	0.18
R289W	0.29	0.21	0.32	0.48	0.20	0.20	0.11
D473W	0.76	0.73	0.67	1.02	0.54	0.48	0.48