TABLE 2.

Dose-response curve parameters for double mutant cycle experiments

IC₅₀ values were determined by fits of data in Fig. 3 to the Hill equation.

Peptide	IC50 (μm)
	αβγ	αD473Wβγ	αR289Wβγ	αQ254Wβγ
LPHPLQRL	0.78 ± 0.09	3.1 ± 0.9	100 ± 10	9 ± 2
LPYPLQRL	2.5 ± 0.2	2.0 ± 0.7	32 ± 8
LPEPLQRL	30 ± 5	39 ± 9	400 ± 20
LPKPLQRL	64 ± 9	50 ± 10
LPHPLQRA	50 ± 30			50 ± 30
LPHPLQRN	56 ± 4			41 ± 6
LPHPLQR_	130 ± 90			100 ± 70