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. 2010 Sep 8;285(45):35224–35237. doi: 10.1074/jbc.M110.165860

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Quantification of Grx1 and Grx2 in bloodstream and procyclic T. brucei. A, representative Western blots of total cell lysates from cultured T. brucei in comparison with different amounts of recombinant Grx1 (bloodstream (BSF) (a) and procyclic (PC) (b) cells) and Grx2 (c). B, the integrated optical densities of each gel band were obtained with the BIO-Profil Biolight program and are depicted versus the protein amount (a, ■; b, ●; c, ▴). C, the cellular concentration of both proteins was derived from the standard lines. The values for Grx1 represent the mean ± S.D. of at least three determinations. In the case of Grx2, the values of two independent analyses are shown.