Figure 1.

Kinetics of base excision by OGG1. OGG1 (1.25 nM) was incubated with varying concentration (3.125–100 nM) of the 8-oxoG·C-containing 32mer duplex oligo for 20 min at 37°C. Reactions were terminated by adding SDS and piperidine to 0.5% and 200 mM respectively, followed by heating at 95°C for 5 min. The products were analyzed as described in Materials and Methods.