Figure 2.

Inhibition of OGG1 glycosylase activity by AP sites. OGG1 (25 nM) was incubated with 500 nM 8-oxoG·C oligo for 5 min at 37°C and the reaction terminated as described in Figure 1. Reaction 1, OGG1 only. Identical reactions were carried out by pre-mixing with 250 nM control G·C oligo (reaction 2), β-elimination product oligo (reaction 3) or AP site-containing oligo (reaction 4).