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. 2001 Jan 15;29(2):430–438. doi: 10.1093/nar/29.2.430

Figure 7.

Figure 7

Kinetics of OGG1, APE1 and Nfo. (A) OGG1 (1.25 nM) and APE1 (6.5 nM) were incubated with increasing amounts of the 8-oxoG·C-containing duplex oligo for 5 min at 37°C. (B) OGG1 (1.25 nM) was incubated with varying amounts (3.125–100 nM) of the AP·C-containing 32mer duplex oligo for 40 min at 37°C. Reactions were terminated by adding SDS and glycerol as in Figure 6B. (C) APE1 (0.5 pM) was incubated with increasing concentrations of the AP·C-containing oligo for 4 min at 37°C. (D) Escherichia coli Nfo (1.25 nM) was incubated with increasing concentrations of the AP·C-containing oligo for 4 min at 37°C. Substrates were in excess and reaction rates were in the linear range in all experiments. Data points are means of three independent experiments.