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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: FEBS Lett. 2010 Aug 6;584(18):3985–3989. doi: 10.1016/j.febslet.2010.08.002

Fig. 3.

Fig. 3

Inhibition of ERK1/2 prevents the hypercapnia-induced AMPK activation. (A) A549 cells transiently transfected with scrambled or ERK 1/2 siRNA and then exposed to 40 (black bars) or 120 (grey bars) mmHg pCO2 for 10 min. Phospho-AMPKα and total amount of AMPKa were measured by Western blot analysis. A representative Western blot is shown for total ERK 1/2. (B) A549 cells transiently transfected with scrambled or AMPKα1 siRNA and then exposed to 40 or 120 mmHg pCO2 for 10 min. Phospho-ERK 1/2 and total amount of ERK 1/2 were measured by Western blot analysis. A representative Western blot of total cell lysates for total AMPKα1 is shown. Data represent the mean ± S.E.M., n = 5. *P < 0.05, **P < 0.01.