Table 2. Primer sequences.
| Gene | Forward primer | MgCl2 [mM] | TAnn [°C] | Size (bp) |
| mir-9-1 | f: GGG AAA TGG GGTATT AGA AAT TTTr: [GGG ACA CCG CTG ATC GTT TA] CAA CAA CAA AAA CCT CAA ACA CPyro: TTT TTG GGT TTG GAT | 1,5 | 60 | 140 |
| mir-9-2 | f: GGA AGA GAT GTT GAT TGA GAA AAr: [GGG ACA CCG CTG ATC GTT TA] TAA TCA ACC AAC TAC CCC ACPyro: GGG ATT GTT GTA ATG TTG | 1,5 | 60 | 114 |
| APC | f: GGA GAG AGA AGT AGT TGT GTA ATT Tr: [GGG ACA CCG CTG ATC GTT TA]A CTA CAC CAA TAC AAC CAC ATA TCPyro: TTA GGG TGT TTT TTA TTT T | 2,5 | 55 | 123 |
| CDH1 | f: AGA TTT TAG TAA TTT TAG GTT AGA GGr: [GGG ACA CCG CTG ATC GTT TA]C TAA TTA ACT AAA AAT TCA CCT ACCPyro a: ATT TTA GGT TAG AGG GTT ATPyro b: TTT GGG GAG GGG TT | 1,5 | 55 | 134 |
| Cyclin-D2 | f: GTA TTT TTT GTA AAG ATA GTT TTG ATTr: [GGG ACA CCG CTG ATC GTT TA] CCA AAC TTT CTC CCT AAA AACPyro: ATA GTT TTG ATT TAA GGA TG | 1,5 | 55 | 117 |
| ESR1 | f: GGY GAG GTG TAT TTG GAT AGT AGr: [GGG ACA CCG CTG ATC GTT TA]C TAT TAA ATA AAA AAA AAC CCC CPyro: GTA TTT GGA TAG TAG TAA GTT | 2,5 | 55 | 208 |
| GSTπ1 | f: GGG GAG GGA TTA TTT TTA TAA Gr: [GGG ACA CCG CTG ATC GTT TA]A ATT AAC CCC ATA CTA AAA ACT CTPyro: GGA TTA TTT TTA TAA GGT | 2,5 | 55 | 173 |
| LINE-1 | n/a (Qiagen) | 1,5 | 50 | n/a |
| MINT31 | f: GTT TAG GGG TGA TGG TTT TAGr: [GGG ACA CCG CTG ATC GTT TA]A AAC ACT TCC CCA ACA TC TACPyro: GTG GTG ATG GAG GTT AT | 1,5 | 55 | 188 |
| RASSF1A | f: AGT TTG GAT TTT GGG GGA GGr: 5′-Biotin-CAA CTC AAT AAA CTC AAA CTC CCCPyro: GGG TTY GTT TTG TGG TTT | 1,5 | 60 | 136 |
| SFRP1 | f: TTG GGG ATT GYG TTT TTT GTTr: [GGG ACA CCG CTG ATC GTT TA] ACT CTA CRC CCT ATT CTC CPyro: GAG GTT TTT GGA AGT TTG | 1,5 | 55 | 108 |
| SOCS1-a | f: GTG AAG ATG GTT TYG GGA TTTr: [GGG ACA CCG CTG ATC GTT TA]C AAC RAA ACC CCC AAC ATA CPyro: TTY GAG TTG TTG GAG TAT TA | 1,5 | 55 | 150 |
| SOCS1-b | f: GTT TTT AGY GTG AAG ATG GTT Tr: [GGG ACA CCG CTG ATC GTT TA] CTA ACR AAA CAA CTC CTA CAA CPyro: GTT TTT ATT TGG ATG GTA G | 1,5 | 55 | 221 |
| univ-bio | 5′-Biotin-[GGG ACA CCG CTG ATC GTT TA] |
Y = Pyrimidine (C/T).
R = Purine (A/G).
f = forward primer.
r = reverse primer.
Pyro = pyrosequencing primer.
Sequences in square brackets resemble universal tag for biotinylated primer.