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. 2010 Oct 29;5(10):e13733. doi: 10.1371/journal.pone.0013733

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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The ESE-Quant Tube scanner equipped with temperature settings to amplify DNA isothermally and spectral devices to detect amplified product using fluorescence is shown (A). The tube scanner can hold 8,200 µL PCR tubes and is equipped with an LCD panel through which positive or negative results can be detected. If the tube scanner is connected to a computer with the appropriate software, the results are obtained in real-time as shown in B. The fluorescence units are shown on the Y-axis and the time to amplification on the x-axis. Amplification curves are observed (solid line) in case of a positive sample. No amplification curves (dotted line) indicate a negative sample.