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. 2010 Oct 29;5(10):e13724. doi: 10.1371/journal.pone.0013724

Table 1. Details of primers used in the study.

Gene Name Sequence (5′….3′) Species Amplicon length Annealing temperature
NADH5 JCND5_159F JCND5_159R CCTATGCCTTTACCATCAGCA GTGCCACGGGAATGAAGAT Jungle cat 98 bp 59°C
JCND5_210F JCND5_210R CTGTGGCACTTTTCGTCA TAAAGCGGCAGTGTTTGC Jungle cat 186 bp 55°C
JCND5_4 F JCND5_4 R ATCCTCTACAACCGCATTGG AGACAGGAGTTGGGCCTTCT Jungle cat and Leopard cat 176 bp 59°C
LepcatND5 F LepcatND5 R GACCCATATATCAACCGA GCGTTTGAGTTAGTAAGG Leopard cat 186 bp 55°C
Cyt b HCJC FHCJC R ATCTCAGCCTTAGCAGCA TTGTCTGGGTCTCCTAGC Jungle cat and Leopard cat 141 bp202 bp 50°C
LepcatCytb2 F LepcatCytb2 R CTGTCTATACATGCACGT TGGCTTTGTCTACTGAGA Leopard cat 239 bp 56°C
LepcatCytb3 F LepcatCytb3 R CATCTTAGGCCTTCTAGT GGAGGATTGGAATGATTG Leopard cat 236 bp 52°C