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. 2010 Oct 29;5(10):e13732. doi: 10.1371/journal.pone.0013732

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Results of two independent experiments were displayed. (A), analysis of expression of Cbx7 in MGC803 cells 72 hours after transfection by Western blot assays. (B), analysis of transcription level of genes located within the p16-Arf-p15 locus, two control genes (Ezh2 and Phc2) located at other loci and three HMTases, in BGC823 cells 72 hours after transfection by quantitative RT-PCR. (C), analysis of transcription level of genes located within the p16-Arf-p15 locus, two control genes (Ezh2 and Phc2) located at other loci and three HMTases, in MGC803 cells 72 hours after transfection by quantitative RT-PCR. P-value less than 0.05 for CBX7/mutants vs. CTRL was listed below each column, respectively. Each column represents the average value of triplicate. The STDEV value was on the top of column. CTRL, cells transfected with the pcDNA3.1(+)/myc-His A control vector; CBX7, cells transfected with the vector containing the full coding region of wildtype Cbx7. ΔPc, Pc-box deleted CBX7; F11A and W32A, CBX7 containing F11A and W32A mutation within chromodomain, respectively.