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. 2010 Oct 29;5(10):e13744. doi: 10.1371/journal.pone.0013744

Figure 2. Integrin subunit mRNAs in mouse oocytes.

Figure 2

RT-PCR analysis of Itgb subunit and selected Itga subunit expression in mouse oocytes (Panel A; lanes 1–22) or control tissues (Panel B; spleen, lanes 23–28, 33–34; liver, lanes 29–30). First strand cDNA was prepared from RNA with reverse transcriptase (+; odd-numbered lanes), or, as a negative control, without RT (−; even-numbered lanes). This shows that oocytes express Itgb1, Itgb3, Itgb5, Itga1, and Itga8 mRNA. Although Itgb2, Itgb4, Itgb6, Itgb7, Itgb8, and Itgad were not detected in oocytes, these PCR products could be amplified from control cDNA.