Figure 2. Epitope density does not prolong CD8⁺ T cell expansion.

A. The number of CD8⁺CD45.2⁺ T cells was quantified over time after immunization with DC O/N and DC 3 h. LNs were sequentially removed to follow T cell response. The number of CD8⁺CD45.2⁺ T cells was normalized to one million cells to correct for LN size variation (relative number). * p<0.05. Results are pooled from 2 independent experiments with 3 mice per group. B. Generation of CD8⁺ Tm cells is also affected by epitope density when naïve T cell precursor frequency is low. 10⁴ OT-I T cells were adoptively transferred. The relative number of Ag-specific T cells recovered over time from the LNs is shown. Results from one representative experiment out of two are shown with 3 mice per group. Statistical analysis was done using a Student's t test.