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. 2010 Oct 29;5(10):e13774. doi: 10.1371/journal.pone.0013774

Figure 3. ATF-3 immunoreactivity in DRG neurons of bone cancer-bearing animals 14 days after implantation into the femur.

Figure 3

(A) Primary ispilateral L3 DRG sub-populations of sensory neurons showing ATF-3-IR-positive neurons marked by dark labeled nuclei with DAB staining (black arrowheads). (B) ATF-3 immunofluorescence labeling was performed to confirm the presence of Fos reactivity (white arrowheads). (C) Immunohistochemistry for ATF3 in the corresponding contralateral DRG. (D) Histogram representation of ATF-3 positive neurons per section in cancer and sham animals. Data are mean ± S.E.M. number of ATF3-LI neurons (n = 24 sections from 3 rats/group; ***: p≤0.001; scale bar: 50 µm).