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. 2010 Oct 29;5(10):e13763. doi: 10.1371/journal.pone.0013763

Figure 2. Rab5 and Rabankyrin5 overexpression increases macropinosome formation.

Figure 2

The distribution of Rab5, Rabankyrin-5 (A) or Rab5(Q79L) (B) in green relative to dextran-positive macropinosomes (red) Scale bar  = 10 µm. (C) HEK-Flp-In cells were transiently transfected with pEGFP-C1, pEGFP-Rab5, pEGFP-Rab5-Q79L and pEYFP-Rabankyrin-5. The mean number of macropinosomes/100 transfected cells was quantitated over 3 replicates of 500 transfected cells for each condition. * denotes statistical significance (p<0.05) using the Student's T-test, performing pairwise analyses relative to cells transfected with pEGFP-C1 alone. Error bars denote S.E.M.