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HEK-Flp-In cells transiently overexpressing pEGFP, pEGFP-SNX18, or pEGFP-ΔSH3-SNX18 were assayed for macropinosome formation as described in Materials and Methods. The mean number of macropinosomes/100 transfected cells was quantitated over 3 replicates of 500 transfected cells for each condition. Error bars denote S.E.M.
