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Subcellular distributions of (a) AT1R, (b) Rab5, and (c) Rab7 in AT1R-HEK293 cells. The AT1R, tagged with EGFP at its C-terminus, is localized mainly at the plasma membrane. Both Rab5-Alexa 546 and Rab7-Alexa 633 are seen as punctuate structures throughout the cytoplasm. (d) overlay of AT1R with DAPI (blue) nuclear stain, (e) overlay of (a) (AT1R-EGFP, green) and (b) (Rab5-Alexa 546, red), and (f) overlay of (a) and (c) (Rab7-Alexa 633, blue). Bar, 10 μm.
