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Internalization of AT1R induced by Ang II (100 nM) treatment for (a–f) 5 min and (g–l) 30 min in AT1R-HEK293 cells. Ang II induced the cellular internalization of (a,g) AT1R, but not (b,h) Rab5 or (c,i) Rab7. (d,j) overlays of AT1R-EGFP (green) and Rab5-Alexa 546 (red); (e,k) overlays of AT1R-EGFP (green) and Rab7-Alexa 633 (blue), and (f,l) overlays of AT1R-EGFP (green), Rab5-Alexa 546 (red), and Rab7-Alexa 633 (blue). Bar, 10 μm.
