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FLIM-FRET analysis of AT1R-EGFP interaction with LAMP1-Alexa 555 in AT1R-HEK293 cells. Cells were treated with (a) vehicle (n=15 cells), Ang II (100 nM) for (b) 5 min (n=8 cells) or (c) 30 min (n=15 cells), as described in Section 2. The AT1R-EGFP lifetime was analyzed in the presence of LAMP1-Alexa 555. Scale bar, 10 μm.
