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Comparison of the AT1R-EGFP lifetimes obtained before and after photobleaching the acceptor, LAMP1-Alexa 555. AT1R-HEK293 cells were treated with Ang II (100 nM) for 30 min, in the presence of LAMP1-Alexa 555 (acceptor). The DPSS 561 laser was used to photobleach the acceptor. Data are mean±S.E.; ∗ P<0.05, Student’s t-test.
